November 13-19, 2011

Ljubljana, Slovenia

Electroporation based Technologies and Treatments

International SCIENTIFIC WORKSHOP and POSTGRADUATE COURSE

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Key lectures and content of the course:

Electrical properties of cells and tissues in electric field

  • Resting and induced transmembrane voltage
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  • Dielectric properties of biological tissues
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  • Temperature and conductivity changes in tissues
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    Physical chemistry of membrane electroporation

  • Electrochemical membrane thermodynamics
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  • Molecular dynamics simulations
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    Electroporation in vitro and in vivo - protocols

  • Electroporation of cells in vitro
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  • Electroporation of cells in vivo
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    Development of devices and electrodes

  • Devices for in vitro and in vivo experimental work
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  • Electrodes for in vitro and in vivo
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  • Electroporation of cells in tissue - models of electroporation in vivo and their experimental validations
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    Electrochemotherapy of tumors

  • Basic mechanisms of electrochemotherapy
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  • Preclinical studies
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  • Clinical use of electrochemotherapy
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  • Current developments and future perspectives
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    Application of electroporation in gene transfection

  • Gene transfection in vitro
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  • Gene transfection in vivo
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  • Clinical use in human medicine
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    Electroporation in transdermal drug delivery

  • Electroporation for gene delivery in the skin
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  • Electroporation for DNA vaccine
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    Additional topics:
  • Vesicles in electric fields, Rumiana Dimova
    		•

  • Pulsed electric field treatment in bioprocessing, Wolfgang Frey
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  • Therapeutic potential of electrically mediated gene transfer and
    results from initial clinical trials, Richard Heller
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  • Endoscopic electroporation for minimally invasive tumor ablation, Declan Soden
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  • Molecular dynamics simulations of lipid bilayers electroporation, Mounir Tarek
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    Laboratory and practical work

  • Gene electrotransfer with different pulse parameters
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  • Monitoring cell membrane electropermeabilization with ratiometric fluorescence dye FURA-2AM
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  • Electropermeabilization detection of attached cells with propidium iodide
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  • Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS
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  • Electroporation of bacteria
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  • Electroporation of planar lipid bilayers
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  • Cell electrofusion
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  • Electrochemotherapy treatment planning: optimization of voltage and electrode position
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  • Numerical modeling of coupled physical phenomena
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  • Molecular dynamics simulations of membrane electroporation
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  • Electrochemotherapy - interactive learning
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