EBTT 2011

The meeting

Cell membrane electroporation — mechanisms & applications

Cell membrane electroporation, its mechanisms and applications in biology, medicine and biotechnology — the basis of the phenomena, current applications and future prospects were the main topics of this international meeting.

The postgraduate course was intended both for novices and experts in electroporation, including PhD students, researchers and users. Abstract submission was encouraged and required where 5 ECTS credits were sought, with each contributor giving a short oral presentation.

Organised by the University of Ljubljana and the Institute of Oncology, Ljubljana.

Course content

Key lectures & topics

Electrical properties of cells & tissues

Resting & induced transmembrane voltage; dielectric properties; temperature & conductivity.

Physical chemistry of electroporation

Electrochemical membrane thermodynamics; molecular dynamics simulations.

Electroporation in vitro & in vivo

Protocols for cells in vitro and in vivo.

Devices & electrodes

Devices and electrodes; tissue models and their validation.

Electrochemotherapy of tumours

Mechanisms, preclinical & clinical use, perspectives.

Gene transfection

Gene electrotransfer in vitro/in vivo; clinical use.

Transdermal drug delivery

Gene delivery in the skin; DNA vaccines.

Additional topics

Vesicles in fields; PEF in bioprocessing; endoscopic ablation; MD simulations.

Faculty

Directors & lecturers

Co-DirectorDamijan Miklavčič

Co-DirectorLluis M. Mir

Faculty members

Damijan MiklavčičUniversity of Ljubljana, Slovenia
Lluis M. MirCNRS & Institut Gustave Roussy, France
Eberhard NeumannUniversity of Bielefeld, Germany
Véronique PréatUC Louvain, Brussels, Belgium
Gregor SeršaInstitute of Oncology, Ljubljana, Slovenia
Justin TeissiéCNRS & IPBS, Toulouse, France
P. Thomas VernierUniversity of Southern California, USA

Invited lecturers

Maja ČemažarInstitute of Oncology, Ljubljana, Slovenia
Rumiana DimovaMax Planck Institute of Colloids & Interfaces, Germany
Wolfgang FreyKarlsruhe Institute of Technology, Germany
Richard HellerUniversity of South Florida, USA
Tadej KotnikUniversity of Ljubljana, Slovenia
Marie-Pierre RolsCNRS & IPBS, Toulouse, France
Declan SodenCork Cancer Research Centre, Ireland
Mounir TarekUniversité Henri-Poincaré, Nancy, France

Hands-on

Practical work & exercises

Wet-lab, computer-modelling and e-learning sessions. Exercise sheets (PDF) as distributed to participants. See also the practical-work assignment form.

S1	Laboratory safety	PDF ↓
L1	Gene electrotransfer with different pulse parameters	PDF ↓
L2	Monitoring cell membrane electroporation with ratiometric fluorescent dye Fura-2AM	PDF ↓
L3	Electropermeabilization detection of attached cells with propidium iodide	PDF ↓
L4	Measurements of the induced transmembrane voltage with di-8-ANEPPS	PDF ↓
L5	Irreversible electroporation of bacterial cells	PDF ↓
L6	Electroporation of planar lipid bilayers	PDF ↓
L7	Cell electrofusion	PDF ↓
C1	Electrochemotherapy treatment planning: optimization of voltage & electrode position	PDF ↓
C2	Numerical modelling of coupled physical phenomena	PDF ↓
C3	Molecular dynamics simulations of membrane electroporation	PDF ↓
E1	Electroporation of cells and tissues — interactive e-learning	PDF ↓

Abstracts & publication

Abstract submission & proceedings

PDFInstructions for Authors PDFAbstract Submission Form DOCAbstract Template PDFJMB Author Guidelines

Participants were encouraged to submit a manuscript for a special issue of the Journal of Membrane Biology devoted to electroporation-based technologies and treatments, guest-edited by P. T. Vernier, L. M. Mir and D. Miklavčič.

Electronic proceedings — all abstracts and course material.

Download proceedings (PDF)

Getting here & staying

Venue & accommodation

Held at the Faculty of Electrical Engineering, University of Ljubljana. Faculty floorplan (PDF) · Map of Ljubljana centre

Hotel Slon

City centre, by Kongresni trg & Restaurant Šestica

Pension Mrak

Near Kongresni trg

University guest house

Dom podiplomcev — Gostišče Jamnik, Cesta na Vrhovce 4

Student dormitory

Dijaški dom Vič, Gerbičeva 51a

Contacts

Organisation & contacts

Registration & scientific programme

Damijan Miklavčič
Faculty of Electrical Engineering, University of Ljubljana
Tržaška 25, 1000 Ljubljana, Slovenia
damijan.miklavcic@fe.uni-lj.si
tel +386 1 4768 456 · fax +386 1 4264 658

Accommodation & travel

Congress Agency Albatros
info@albatros-bled.com
tel +386 4 5780 358 · fax +386 4 5780 355